Jump to Navigation

New equipment for bidimensional electrophoresis (2DE)


New equipment for bidimensional electrophoresis (2DE)

In bidimensional electrophoresis, proteins are separated according to two independent properties in two discrete steps: (1) Isoelectric focusing (IEF), which separates proteins according to their isoelectric points (pI), and (2) SDSpolyacrylamide gel electrophoresis (SDS-PAGE), which separates them according to their molecular weights (MW). At this stage, the gels are scanned by a laser scanner with high sensitivity and accuracy. Ettan Spot Picker is a robotic system designed to accurately collect protein spots from 2-D gels for further analyses, such as digestion and protein identification by MALDI mass spectrometry (see details in annex).

The power of 2-DE as a biochemical separation technique has been recognized since its introduction in 1975. Its application, however, has become increasingly significant as a result of a number of developments in separation techniques, image analysis, and protein characterization. Applications include: proteome analysis, cell differentiation
studies, detection of disease markers in research, cancer research, etc.

Equipments aqcuired:

  • Ettan IPGphor 3 for first dimension (isoelectric focusing) is a fully integrated isoelectric focusing (IEF) system that allows proteins to be separated according to their
    isoelectric points.
  • Ettan DALTsix electrophoresis Unit for second dimension (vertical electrophoresis)
  • Laser Scanner Typhoon FLA 9500, for gel scanning, will be used to scan the 2DE gels
  • Ettan Spot Picker: automatic excision of the protein spots
  • Amersham Imager 600 is a sensitive and robust chemiluminescence imager for high-resolution digital imaging of protein in gels and membranes (western blotting). Images can be afterward analyzed with the IMageQuant TL software to perform protein quantification, ensuring accurate and consistent results.



Main menu 2

Page | by Dr. Radut